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Foreign DNA in mammalian systems by Walter Doerfler

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Published by Wiley-VCH in Weinheim, New York .
Written in English


  • Genetic transformation.,
  • Transfection.,
  • Mammals -- Genetics.,
  • Adenoviruses.,
  • DNA.

Book details:

Edition Notes

Includes bibliograhical references and index.

StatementWalter Doerfler.
LC ClassificationsQH448.4 .D64 2000
The Physical Object
Paginationxiii, 181 p. :
Number of Pages181
ID Numbers
Open LibraryOL3962835M
ISBN 103527300899
LC Control Number2001266996

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Request PDF | Foreign DNA in Mammalian Systems | It is unlikely that the established genomes of present day organisms remain stable forever. It . Get this from a library! Foreign DNA in mammalian systems. [Walter Doerfler] -- "The established genomes of today's organisms are unlikely to remain stable forever. Foreign DNA can gain access to individual cells of an organism of the same or a different species. The natural. Comparisons with other viral systems -- 8. Non-viral systems -- 9. Patterns of DNA methylation in the human and in viral genomes -- Consequences of foreign DNA integration and persistence -- Uptake of foreign DNA from the environment: the gastrointestinal tract and the placenta as portals of entry -- Summary This chapter contains sections titled: Foreign DNA in the environment Uptake and modes of persistence of foreign DNA in mammalian cells Further reading Foreign DNA - Foreign DNA in Mammalian Systems - Wiley Online Library.

  Foreign DNA in Mammalian Systems by Walter Doerfler, , available at Book Depository with free delivery : Walter Doerfler. Adenoviral Vector Construction I: Mammalian Systems 7 9 the foreign DNA and the kanamycin resistant gene is then cloned into the Pad site of the pBHG plasmid. Following transformation of E. coli, positive clones bearing the E3 insertion are easily identified by their resistance to both ampicillin and by: 4. The fate of foreign DNA in mammalian cells and organisms Article in Developments in biologicals ; discussion February with 85 Reads How we measure 'reads'. In this article, two intertwined observations on the fate of foreign DNA in mammalian systems and their combined impact on pathogenesis have become the basis for a new hypothesis. 1. The invasion of foreign DNA into mammalian cells, particularly in its genomically integrated configuration, can lead to genome-wide alterations of the cells.

  The fate of foreign DNA in mammalian systems has been a long-term interest in our laboratory [Doerfler (Foreign DNA in mammalian systems. Wiley-VCH, )]. The current overview summarizes an update of the data presented at the Fifth Weissenburg Symposium (Weissenburg Symposia, –).Cited by: 2. Restriction enzymes to create fragments with sticky ends and DNA ligase to covalently link them allow foreign DNA to be inserted into plasmid vectors in vitro in a straightforward procedure. E. coli plasmid vectors can be constructed with a polylinker, a synthetic multiple-cloning-site sequence that contains one copy of several different Cited by: 5.   BOOK REVIEW. Abstract. Naturwissenschaften () Q Springer-Verlag Foreign DNA in Mammalian Systems, Walter Doerfler, tims. Although not intended as such, Foreign DNA in ; 1st edn XIII; pages; hard cover, ISBN Mammalian Systems, by avoiding confusion through , DM 98, Wiley VCH Weinheim . ADVANCES IN CELL CULTURE, VOL. 2 INTRODUCTION AND EXPRESSION OF FOREIGN DNA SEQUENCES IN MAMMALIAN CELLS Raju Kucherlapati1 Department of Biochemical Sciences Princeton University Princeton, New Jersey Introduction Methods of Introducing Genes A. Classical Methods B. DNA Transfer Systems III. Vectors for Introduction of DNA by: 9.